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Faculty of Medicine
Document Details
Document Type
:
Article In Journal
Document Title
:
Preliminary characterization of a placental factor inhibiting breathing in fetal sheep.
Preliminary characterization of a placental factor inhibiting breathing in fetal sheep.
Subject
:
Neonatology
Document Language
:
English
Abstract
:
Previous studies have revealed a placental extract that inhibits breathing in fetal sheep. In the present study of 29 chronically instrumented sheep at 132±1 days of gestation, infusion of the 1-10 kDa extract inhibited breathing in 76% of the experiments whereas Krebs’ solution inhibited it in 24%. It retained this activity after 6 months of freezing, after lyophilization, and upon lowering the pH during purication from 8·0 to 4·0, but it inhibited breathing in only 35% when the pH was lowered to 2·0. A signicant dose-dependent effect was observed from a 16-fold dilution to a 4-fold concentration. Treatment of the extract with proteinase K or boiling reduced the activity to 30% or 26% inhibition, respectively. The activity was not adsorbed to an ion-exchange column at pH 7·0 or 8· 0, but it was at pH 9· 0 and it eluted with increasing NaCl concentrations. On a polyacrylamide gel the activity was eluted at a Kav of 0· 66 (82% inhibition), corresponding to between 2·5 and 4·5 kDa. These ndings suggest that a peptide produced by the placenta, with a molecular mass between 2· 5 and 4·5 kDa, inhibits fetal breathing
ISSN
:
1031-3613
Journal Name
:
Reproduction, Fertility and Development
Volume
:
9
Issue Number
:
6
Publishing Year
:
1417 AH
1997 AD
Article Type
:
Article
Added Date
:
Monday, August 31, 2009
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
سعد الصاعدي
Al-Saedi, Saad
Researcher
Doctorate
Files
File Name
Type
Description
29349.zip
zip
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