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Faculty of Science and Arts - Khulais
Document Details
Document Type
:
Article In Journal
Document Title
:
Chemical and acidic denaturation of a homodimeric glutathione transferase mu class from Rhipicephalus (Boophilus) annulatus
التفكيك الكيميائى و الحامضى لإنزيم الجلوتاثيون ترانسفيريز ثنائى التجانس من العائلة ميو المنقى من قراد البقر ريبيسيفيلاس (بوؤفيلس) أنيولاتس.
Subject
:
Enzymology
Document Language
:
English
Abstract
:
The equilibrium unfolding of a mu class glutathione transferase from Rhipicephalus (Boophilus) annulatus (BaGSTM) has been performed using guanidinium chloride (GdmCl), urea and acid denaturation to investigate the unfolding intermediates. Protein transitions were monitored by intrinsic fluorescence and 8-anilino-1-naphthalenesulfonate (ANS) binding. The results indicate that unfolding of BaGSTM using GdmCl (0-4.0 M) is a multi-step process, i.e., at least two intermediates coexist in equilibrium. The first intermediate, a partially dissociated dimer, exists at low GdmCl concentration (less than 1.5 M). This intermediate undergoes dissociation into two monomers at GdmCl concentration between 1.5 and 2.0 M. The monomeric intermediate started to be completely unfolded at higher GdmCl concentrations (> 2.0 M). Unfolding using urea (0-8.0 M) and acid-induced structures as well as the ANS fluorescence in presence of different concentrations of GdmCl or urea confirmed that the unfolding is a multi-step process. The formation of a molten globule state (a monomeric intermediate) at pH less than 3.8 was suggested by the strong enhancement of fluorescence of ANS and protein concentration dependent.
ISSN
:
1687-157X
Journal Name
:
J. of Genetic Eng. & Biotechnol
Volume
:
6
Issue Number
:
1
Publishing Year
:
1429 AH
2008 AD
Article Type
:
Article
Added Date
:
Saturday, January 15, 2011
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
عمرو السيد الحكيم
El Hakim, Amr El Sayed
Researcher
Doctorate
aselhakim@hotmail.com
ياسر عزت شاهين
Shahein, Yasser Ezzat
Researcher
Doctorate
رجاء رضا حامد
Hamed, Ragaa Reda
Researcher
Doctorate
عبد المنعم عبد الحميد عبد الله
Abd Allah, Abdul Monem Abdel Hamid
Researcher
Doctorate
يسري محمد حمدي
Hamdey, Yosery mohamed
Researcher
Doctorate
Files
File Name
Type
Description
28631.pdf
pdf
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