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Faculty of Medicine
Document Details
Document Type
:
Article In Journal
Document Title
:
Comparison of fluorescence polarization immunoassay and high performance liquid chromatography for the quantitative determination of phenytoin, phenobarbitone and carbamazepine in serum
Comparison of fluorescence polarization immunoassay and high performance liquid chromatography for the quantitative determination of phenytoin, phenobarbitone and carbamazepine in serum
Subject
:
Pharmacology
Document Language
:
English
Abstract
:
The Abbott TDx fluorescence polarization immunoassay (FPIA) system has been evaluated and compared with well-established high performance liquid chromatography (HPLC) for the determination of three anticonvulsant drugs: phenytoin, phenobarbitone and carbamazepine. These assays were evaluated for precision, calibration curve stability, specificity and accuracy. Within-run precision studies using control samples (n = 15) in the subtherapeutic, therapeutic, and toxic concentrations, resulted in coefficients of variation in the range of 1.79-3.99% (FPIA) and 1.16-2.52% (HPLC), respectively. Between-run precision ranged from 2.32-6.34% for FPIA and from 2.04-3.38% for HPLC. Comparison of 122 patient samples assayed with both methods indicated an extremely good analytical correlation (r = 0.96) for all three comparisons. The FPIA method offers significant advantages in calibration curve stability while maintaining accuracy and precision comparable with those of established HPLC procedures
ISSN
:
0022-3573
Journal Name
:
Journal of Pharmacy and Pharmacology
Volume
:
38
Issue Number
:
8
Publishing Year
:
1406 AH
1986 AD
Article Type
:
Article
Added Date
:
Thursday, November 24, 2011
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
عبدالرحمن آشي
Ashy, AbdulRahman
Investigator
Doctorate
سميرة اسلام
Islam, Samira
Researcher
Doctorate
Files
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Type
Description
31322.pdf
pdf
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