Document Details

Document Type : Article In Journal 
Document Title :
ii-Preliminary Study in Diagnosis and Early Prediction of Preeclampsia by Using FTIR Spectroscopy
ii-Preliminary Study in Diagnosis and Early Prediction of Preeclampsia by Using FTIR Spectroscopy
 
Subject : Biochemistry 
Document Language : English 
Abstract : Preeclampsia is a heterogeneous condition, potentially involving several separate pathophysiological pathways; currently no clinical screening test is useful for prediction of preeclampsia development. Fouriertransform infrared spectroscopy (FTIR) holds great promise for clinical chemistry measurements. FTIR spectra of plasma samples from pregnant women -14 patients and 31 normotensive were obtained. Second derivative spectra, Kramer Krong refractive index and ANOVA test were tacking in comparison studies. The parameters studied were proteins and lipids. Different absorbance ratios for specific bands were calculated and plotted versus the patient samples. The absorbance IR spectra of these two groups were slightly different, but from the curve fitting analysis, the protein secondary structure compositions were significant different. The decrease in á-helix structure due to oxidative stress in patient group might be responsible of the dramatic increase in â- turns and unordered structure. Moreover, the peaks present in the IR second derivative, for patient group, at 1744cm-1 (cholesterol and triglycerides ester C=O), 1710cm-1 (carbonyl C-O stretch), and 1621cm-1 (peptide C=O stretch) positively correlated with low density lipoprotein (LDL) oxidation. The results showed that among the normotensive control group three subjects later developed preeclampsia. Normotensive pregnant women who developed preeclampsia were considered as subjects at high risk. This study suggests, for the first time that FT-IR spectroscopy can be successfully used as an accurate and rapid test, for diagnosis and confirmed with 33% confidence level early prediction of preeclampsia, starting from 20 week of gestation. 
ISSN : 1545-1003 
Journal Name : Journal of American Science 
Volume : 7 
Issue Number : 4 
Publishing Year : 1432 AH
2011 AD 
Article Type : Article 
Added Date : Monday, December 26, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
جيهان عبدالرؤوفA. Raouf, Gehan ResearcherDoctorategehan_raouf@hotmail.com
عبدالرحمن لبيد المالكيAl-Malki, Abdulrahman LResearcherDoctoratealalmalki@kau.edu.sa
نسمه منصوريMansouri, Nesma ResearcherDoctoratenmansouri@kau.edu.sa
رقية محمد المحموديAl-Mahmoudi, Rogaia MResearcherMaster 

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