Document Details

Document Type : Article In Conference 
Document Title :
Histomorphometric Assessment of Bony Healing of Rabbit Critical-Sized Calvarial Defects With Hyperbaric Oxygen Therapy
القياس النسيجي لتقييم شفاء العظم من العيوب ذات الحجم الحرج مع العلاج بالأكسجين عالي الضغط
 
Subject : Oral and Maxillofacial surgery 
Document Language : English 
Abstract : Statement of the Problem: A critical-sized defect is the smallest osseous wound that will not heal spontaneously with bony union over the lifetime of an animal. Practically, the defect should not heal spontaneously during the experimental period. Hyperbaric oxygen therapy (HBO) is used to improve the healing of a variety of problem wounds. This study evaluated the effect of HBO on the healing of critical-sized defects in the rabbit calvarial model and whether HBO administration can result in the healing of a larger “supracritical-sized” defect. Materials and Methods: Twenty New Zealand rabbits were divided into 2 groups of 10 animals. Full thickness calvarial defects were created in their parietal bones bilaterally. Defects were critical-sized (15 mm) on one side and supracritical (18 mm) on the contralateral side. Group 1 received a 90 minute HBO therapy session at 2.4 ATA daily for 20 consecutive days. Group 2 served as a control group receiving only room air. Five animals in each group were sacrificed at 6 and 12 weeks postoperatively. Method of Data Analysis: Data analysis included qualitative assessment of the calvarial specimens as well as quantitative histomorphometric analysis to compute the amount of regenerated bone within the defects. Hematoxylin and eosin stained sections were sliced and captured by a digital camera (RT Color; Diagnostic Instruments Inc, Sterling Heights, MI). A blinded investigator examined merged images and analyzed them for quantity of new bone regeneration. Statistical significance was established with a p value <.05. Results: The HBO group showed bony union and demonstrated more bone formation than the control group at 6 weeks (p < .001). The control group did not show bony union in either defect by 12 weeks. There was no significant difference in the amount of new bone formed in the HBO group at 6 weeks compared with 12 weeks (p  .309). However, the bone at 6 weeks was more of a woven character, while at 12 weeks it was more lamellated and more mature. Again, in the HBO group both the critical-sized and the supracritical-sized defects healed equally (p = .520). Conclusion: HBO therapy has facilitated the bony healing of both critical-sized and supracritical-sized rabbit calvarial defects. Since bony healing was achieved early, it is reasonable to assume that an even larger than 18 mm defect (if it were technically feasible) might have healed within the 12 week period of study aided by HBO. Adjunctive HBO, based on histomorphometrics, doubles the amount of new bone formed within both the critical sized and the supracritical-sized defects. It allowed an increase in the critical size by more than 20%. 
Conference Name : American Association of Oral and Maxillofacial Surgeons 
Duration : From : 24/08/1426 AH - To : 28/08/1426 AH
From : 20/09/2005 AD - To : 24/09/2005 AD 
Publishing Year : 1426 AH
2005 AD 
Number Of Pages : 2 
Article Type : Article 
Conference Place : Boston, USA 
Organizing Body : American Association of Oral and Maxillofacial Surgeons 
Added Date : Thursday, August 9, 2012 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
أحمد محمد سعيد جانJan, Ahmed Mohammed SaeedInvestigatorDoctorateamjan@kau.edu.sa

Files

File NameTypeDescription
 34086.pdf pdfAhmed Jan Histomorph JOMS 2005

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