Document Details
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Document Type |
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Thesis |
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Document Title |
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Comparative Physiological Studies of Brassica nabus L. ( canola ) and Schouwia purpuria L. (Forssk) subsp. Arabica plants and their sign of heat tolerance دراسة مقارنة فسيولوجية لنباتي الكانولا والشوايا بوربوريا البري ومؤشرات تحملهما للحرارة |
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Document Language |
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Arabic |
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Abstract |
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) The aim of this work is to study the mechanism of Canola and Schouwia purpurea plants to tolerate high temperature when subjected to elevated change in temperature for different periods of time and how these plants adapted to these new conditions .
In the present study, the effect of temperature stress was carried on two plant species from the family cruciferae , Brassica napus ( canola) , and Schouwia purpurea . The plant seedlings were subjected to two ranges of temperature 35˚ and 45 °C for different time (1, 2, 8, 24, 48 hours ) and 7 days . Pigments and carbohydrate contents were estimated colorimetriclly , while proteins and esterase enzyme were separated by gel electrophoresis .
Estimation of pigment contents of canola plants revealed a significant effect on chlorophyll a, chlorophyll b and carotenoid contents when subjected to 2 and 8 hours. On the other hand, prolonged time of exposure 24 hrs. for 7 days showed no significant effects on the previous estimation. No significant effects were observed for Schouwia purpurea .
In general, reduction in reducing sugar contents were observed in canola plants after exposure to 35˚, 45˚ C compared to control plants . Treatment of the plants with 45° C for 2 hours showed a remarkable in the reducing sugar contents .
For Schouwia purpurea , prolonged exposure to temperature (1, 2, 8 and 24 h) resulted in a reduction of reducing sugars , while the increase of those sugars was observed when these plants exposed to 48 h and 7 days at 35° C and 45° C .
In canola plants , elevated temperature reduced polysaccharide accumulation compared to control . In Schouwia purpurea , starch contents increased in plants treated with 35˚ C for 1,2,8 and 48 hours and to 45˚ C for 2 and 24 hrs . Low accumulation of starch was detected after 7 days of treatment .
Finally, in the effect of temperature on firmness protein has been observed that the highest increase the number of protein bands was when treated Canola plants for 8 hours at temperature of 35 ˚ C as well as after treatment for 2 hours at a temperature of 45 ˚ C. As for the Schouwia purpurea was the highest increase at a temperature of 35 ̊ C for ( 48 hours and 7 days) also at a temperature of 45 ̊ M for ( 8, 48 hours and 7 days).
On other hand the temperature of esterase enzyme in Canola plants was 35 ˚ C that was less impact on the disappearance of alleles compared to the treatment of these plants at a temperature 45 ˚ C .As for in Schouwia purpurea plants did not note any effect upon the activity of the enzyme at higher temperature. |
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Supervisor |
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Prof. Dr. Salih Ali Ba-Zaid |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1432 AH
2011 AD |
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Number Of Pages |
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88 |
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Added Date |
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Saturday, May 19, 2012 |
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Researchers
| سلمى حامد السلمي | Al-Sulamy, salma Hamed | Researcher | Master | Lotus.flower_s@hotmail.com |
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